Table 1 Candidate reference genes and primer sequences
Gene symbol | Gene Name | GenBank Accession | 5'-Primer Sequences (Forward/Reverse)-3' | Product size (bp) |
|---|---|---|---|---|
Actb | Actin beta | NM_007393 | GGCTGTATTCCCCTCCATCG / CCAGTTGGTAACAATGCCATGT | 154 |
Gapdh | Glyceraldehyde-3-phosphate dehydrogenase | NM_001289726 | AGGTCGGTGTGAACGGATTTG / TGTAGACCATGTAGTTGAGGTCA | 123 |
Reep5 | Receptor accessory protein 5 | NM_007874 | GGTTCCTGCACGAGAAGAACT / GAGAGAGGCTCCATAACCGAA | 140 |
Rpl5 | Ribosomal protein L5 | NM_016980 | TTGGTGATCCAGGACAAGAATAA / GCACAGACGATCATATCCCC | 125 |
Psmb4 | Proteasome subunit beta 4 | NM_008945 | ATGGAAGCGTTTTGGGAGTCA / GTTCTGGGTCCGAGTGATGG | 144 |
Vcp | Valosin containing protein | NM_009503 | GCTTGTAAACTGGCCATTCG / GATCTCAGGCACTGGATCGT | 114 |
B2m | Beta-2-microglobulin | NM_009735 | TTCTGGTGCTTGTCTCACTGA / CAGTATGTTCGGCTTCCCATTC | 104 |
Gusb | Glucuronidase beta | NM_010368 | GGCTGGTGACCTACTGGATTT / GGCACTGGGAACCTGAAGT | 131 |
Hmbs | Hydroxymethylbilane synthase | NM_001110251 | AAGGGCTTTTCTGAGGCACC / AGTTGCCCATCTTTCATCACTG | 78 |
Hprt1 | Hypoxanthine phosphoribosyltransferase 1 | NM_013556 | GGTTAAGCAGTACAGCCCCA / GGCCTGTATCCAACACTTCG | 81 |
Ipo8 | Importin 8 | NM_001081113 | ACGTGACAGTAGATACCAACGC / GCATAGCACTCGGCATCTTCT | 115 |
Pgk1 | Phosphoglycerate kinase 1 | NM_008828 | ATGTCGCTTTCCAACAAGCTG / GCTCCATTGTCCAAGCAGAAT | 164 |
Polr2a | RNA polymerase II subunit A | NM_001291068 | AAATACCCAGAAACAACGGAGG / CCAGTCCGCTCAATCACCC | 83 |
Ppia | Peptidylprolyl isomerase A | NM_008907 | GAGCTGTTTGCAGACAAAGTTC / CCCTGGCACATGAATCCTGG | 125 |
Rplp0 | Ribosomal protein lateral stalk subunit P0 | NM_007475 | AGATTCGGGATATGCTGTTGGC / TCGGGTCCTAGACCAGTGTTC | 109 |
Tbp | TATA box binding protein | NM_013684 | GTGGGGAGCTGTGATGTGA / TCCAGGAAATAATTCTGGCTCA | 96 |
Tfrc | Transferrin receptor | NM_011638 | GTTTCTGCCAGCCCCTTATTAT / GCAAGGAAAGGATATGCAGCA | 152 |
Ubc | Ubiquitin C | NM_019639 | GAGGTGGCATGCAGATCTTT / CCCTCCTTGTCCTGGATCTT | 112 |
Ywhaz | Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta | NM_011740 | GAAAAGTTCTTGATCCCCAATGC / TGTGACTGGTCCACAATTCCTT | 134 |
18S | eukaryotic 18S ribosomal RNA | NR_003278 | CTCAACACGGGAAACCTCAC / CGCTCCACCAACTAAGAACG | 110 |
Sdha | succinate dehydrogenase complex flavoprotein subunit A | NM_023281 | GGAACACTCCAAAAACAGACCT / CCACCACTGGGTATTGAGTAGAA | 106 |