Fig. 4
From: Development of the indirect flight muscles of Aedes aegypti, a main arbovirus vector
F-actin and myosin filaments patterns in DVM primordia. L4 DVM primordia obtained from L4 instar was stained with TRITC-Phalloidin (red), DAPI (blue), and anti-Myosin Heavy Chain antibody (MHC) (green) to observe the F-actin and nuclei distribution in forming myofilaments. Images of optical slices were collected by confocal microscopy and a projection constructed using the ZEN program (Zeiss). a A structure showing hundreds of immature myofibrils forming five possible muscle fibers (1–5, clearly separated). The DVM primordium showed an approximate size of 300 μm in both length and width. b Nuclei forming rows within the primordium. c The same primordium showing hundreds immature myofibrils formed by F-actin whitin muscle fibers. Also, both ends of primordium were intensely stained with actin. d Myosin distribution in primordium shows distribution parallel to actin. d Merge. Scale bars: b–e 100 μm