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Fig. 5 | BMC Developmental Biology

Fig. 5

From: Wdpcp regulates cellular proliferation and differentiation in the developing limb via hedgehog signaling

Fig. 5

Loss of Wdpcp disrupts osteogenic differentiation. A, B Alizarin Red staining for mineralized matrix of control versus Wdpcp-cKO mesenchymal progenitor cells cultured in osteogenic medium for 20 days. C Alkaline phosphatase (ALP) activity in control versus Wdpcp-cKO mesenchymal progenitor cells cultured in osteogenic medium for 4 days. D Hedgehog pathway activity as assayed by expression of hedgehog reporter genes Gli1 and Ptch1 of control versus Wdpcp-cKO mesenchymal progenitor cells cultured in osteogenic medium for 20 days. E Expression of early osteogenic differentiation marker Runx2 and mature osteogenic markers Osx, Col1a1, and Ocn in control versus Wdpcp-cKO mesenchymal progenitor cells cultured in osteogenic medium for 20 days.. Error bars represent one standard deviation. Effect of drug treatment within a genotype is analyzed using Student’s t-test with * for p < 0.05, ** for p < 0.01, *** for p < 0.001

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