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Fig. 2 | BMC Developmental Biology

Fig. 2

From: Wdpcp regulates cellular proliferation and differentiation in the developing limb via hedgehog signaling

Fig. 2

Developing limbs of Wdpcp-cKO embryos have smaller appendicular skeletal cartilage anlagen, delayed expression of hypertrophic chondrocyte markers and expanded hypertrophic zones in growth plate. A-C Alcian Blue and Alizarin Red staining of E14.5 embryos with quantification of anlagen lengths. D, E Immunohistochemistry staining of E14.5 humerus for chondrocyte hypertrophy markers Runx2 and Sp7. F, G Safranin O Fast Green staining of P0 growth plates and (H) quantification of growth plate zone sizes. Scale bars are 1 mm for (A, B), and 50 μm for (D-G). Error bars represent one standard deviation. Wdpcp-cKO is compared to matched control with * for p < 0.05, ** for p < 0.01, and *** for p < 0.001

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