Fig. 5

Differential Apela and Apelin expression in the endoderm. (a): the ‘top’ and ‘bottom’ parts of dissected embryos used for single-cell transcriptomics. (b): Expression of Tal1, Sox17, Flk1 and Brachyury (T) mapped onto t-distributed stochastic neighbour embedding (t-SNE) plots, showing the aggregate of all cells of ‘top’ and ‘bottom’ parts (as shown in a) of twenty-five (E7.25-E7.5) embryos in grey, the localisation of gene expression in the cells of the ‘top’ part in red and of the ‘bottom’ part in blue. The intensity of the colour represents the expression levels of the genes in these cells. (c): Secreted factors that are at least two-fold differentially expressed between Sox17 expressing cells of the ‘top’ sample versus the ‘bottom’ sample. The difference in fold expression is indicated between brackets. In green are indicated the secreted factors for which the receptor is expressed in Tal1+ cells