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Fig. 7 | BMC Developmental Biology

Fig. 7

From: Natriuretic peptides and Forkhead O transcription factors act in a cooperative manner to promote cardiomyocyte cell cycle re-entry in the postnatal mouse heart

Fig. 7

Cardiomyocyte cell cycle activity with ANP and DN-FOXO treatment in vivo. a Study design of ANP and DN-FOXO treatment by intramyocardial injection after MI by ligation of the left anterior descending coronary artery. b Confocal images showing cardiomyocytes in DNA synthesis-phase visualized using Click-iT EdU Alexa Fluor (green) and co-immunostaining with the antibody against cTnT (red) in sectioned heart ventricles at P8. Arrows point to EdU+cTnT+ cells. Cardiomyocyte boundaries were visualized using WGA staining (white). Scale bars: 20 μm. c Quantification of EdU+cTnT+ cells as a percentage of total cTnT+ cells analyzed on heart ventricular sections from P8 hearts (n=3–5 per group, ~ 1000 cTnT+ cells per sample). d Quantification of EdU+cTnT- cells as a percentage of total cTnT- cells analyzed on heart ventricular sections from P8 hearts. e Quantification of cell apoptosis as the number of TUNEL+ cells on heart ventricular sections per field. P value was calculated using one-way ANOVA. ***P < 0.001; ****P < 0.0001; n.s. = Not significant

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