Skip to main content
Fig. 6 | BMC Developmental Biology

Fig. 6

From: Natriuretic peptides and Forkhead O transcription factors act in a cooperative manner to promote cardiomyocyte cell cycle re-entry in the postnatal mouse heart

Fig. 6

Enhanced cardiomyocyte cell cycle activity observed in the simultaneous treatment of natriuretic peptide and DN-FOXO was primarily mediated through Npr3. a qRT-PCR analysis showing expression of Npr1, Npr2, and Npr3 in cultured neonatal mouse cardiomyocytes at 48 h after infection with lentivirus expressing either Npr1 shRNA, Npr2 shRNA, or Npr3 shRNA. b and c Npr3 shRNA treatment abrogating the effects of ANP/BNP and DN-FOXO effects on cardiomyocyte cell cycle activity. Quantification of cardiomyocyte cell cycle activity as the percentage of Ki67+cTnT+ (b) and EdU+cTnT+ cells (c) of total cTnT+ cells analyzed. n=4–5 per group. P value was calculated using one-way ANOVA. **P < 0.01; ***P < 0.001; ****P < 0.0001; n.s.= Not significant

Back to article page