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Fig. 5 | BMC Developmental Biology

Fig. 5

From: Natriuretic peptides and Forkhead O transcription factors act in a cooperative manner to promote cardiomyocyte cell cycle re-entry in the postnatal mouse heart

Fig. 5

Cardiomyocyte cell cycle activity with natriuretic peptide and DN-FOXO treatment in vitro. a Isolated neonatal mouse cardiomyocytes (P1) were cultured with a medium containing Ad-Cre, ANP, BNP, or Ad-DN-FOXO for 48 h. Cardiomyocyte cell cycle activity was visualized by co-immunostaining for Ki67 (red) and cTnT (green) on cultured cardiomyocytes. Arrows point to Ki67+cTnT+ cells. b Quantification of Ki67+cTnT+ cells as a percentage of total cTnT+ cells analyzed per field (~ 1000 cTnT+ cells per sample). c Cardiomyocytes in the DNA synthesis-phase were detected by using Click-iT EdU Alexa Fluor (red) and co-immunostaining with the antibody against cTnT (green) on tissue sections. Arrows point to EdU+cTnT+ cells. d Quantification of EdU+cTnT+ cells as a percentage of total cTnT+ cells analyzed per field (~ 1000 cTnT+ cells per sample). Scale bars: 50 μm. Data are mean ± s.e.m. P value was calculated using one-way ANOVA. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; n.s.= Not significant

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BMC Developmental Biology

ISSN: 1471-213X

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