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Fig. 6 | BMC Developmental Biology

Fig. 6

From: Dynamic conversion of cell sorting patterns in aggregates of embryonic stem cells with differential adhesive affinity

Fig. 6

Formation of polarized surface is independent of endoderm differentiation in ES cell aggregation. a Spheroids from the 24- and 48-h aggregation of wild-type ES cells were analyzed for confocal immunostaining of Actin and Dab2 (a marker for endoderm differentiation). Individual spheroids are also shown at higher magnification. An arrowhead indicates surface cells with uniformly distributed actin at 24-h time course. An arrow indicates polarized actin cap on the apical domain of surface cells that are undifferentiated (Dab2-negative). In a rare spheroid containing differentiated cells, the differentiated cells positioned on the surface lack actin cap, as indicated by an arrowhead. b Confocal images of spheroids from aggregation of wild-type intermixed with E-cadherin deficient ES cells are shown for Actin and Dab2 immunostaining. Individual spheroids presented at a higher magnification show uniform Actin staining at 24 h (arrowhead), and polarized and undifferentiated superficial cells harboring actin cap at 48 h (arrow). Rare spheroids containing differentiated cells also show an actin cap on the apical surface of undifferentiated surface cells (arrow)

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