Fig. 1

Construction and identification of a Conditional Targeting Vector For PSMD11. a Schematic figures of the wild-type allele of PSMD11, the targeting vector, PSMD11^neo-flx (recombinant allele), PSMD11^flx(conditional floxed allele after Flp recombination) and PSMD11^∆(constitutive KO allele after Cre recombination) alleles. Blue boxes, Brown triangles and purple triangles represent exons with the exon number indicated, loxP sites and frt sites separately. The 5′ long homology arm (LA) including exon 4 of the targeting vector is∼4.93 kb, the 3′ short homology arm (SA) is∼2.85 kb. Small Red line denote the location of Neo probe for Southern blot. Arrows show the location of the primers for sequencing and genotyping. The F1 PSMD11 ^flx-neo/+ mice was crossed with the FLPe deleter mice to remove the Neo cassette in the germline. PSMD11^flx/flx mice were mated with CMV-Cre mice to generate PSMD11^∆/+ mice. b Comparison of mRNA transcripts from PSMD11 exon 5 floxed allele or deleted allele to show the introduction of a stop codon (*) after removal of the E5 in the chimeric transcript of E4 and E6. The predicted amino acids are shown below the mRNA labeled in colors. c and d Restriction endonuclease pattern and analysis of the targeting vector which was digested with restriction endonuclease ApaLI, DrdI, EcoRI, FspI5 or NotI respectively, the DNA fragments was separated by 0.5% gel electrophoresis