Fig. 6

cGMP immunostaining in Pacific oyster larvae (a-f) and spat (g-k). Fluorescent and bright-field images of whole-mount stained individuals: 16 dpf larva (A, flattened with cover slip), 17 dpf larvae (c), 17 dpf larva 3 hpe EPI (e), spat with visible foot remnants (g), spat without visible foot remnants (j); fluorescent images of sectioned individuals accompanied with superimposed DAPI/cGMP signals as well as H&E staining: transverse section 17 dpf larva (b), sagittal section 17 dpf larva (d), frontal section 17 dpf larva 6hpe EPI (f), sagittal sections spat with large (h) and small foot remnants (k). e: oesophagus; f: foot; fg C: foot glands C; fg D: foot glands D; g: gills; gr: gill rudiments; m: mantle; pam: posterior adductor muscle; pg: pedal ganglia; r. f: remnants foot; r. fg C: remnants foot glands C; ve: velum. Scale bar: 50 μm