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Fig. 7 | BMC Developmental Biology

Fig. 7

From: Transcriptomic profiles of non-embryogenic and embryogenic callus cells in a highly regenerative upland cotton line (Gossypium hirsutum L.)

Fig. 7

Evaluation of gene expression in embryogenic cells (EC) and non-embryogenic cells (NEC) by RT-qPCR analysis. Five of the selected genes were up-regulated (left); and the other five genes were down-regulated in embryogenic cells (right). GhPP2A1 and GhUb7 were used as the internal controls. Three biological replicates and three technical replicates were used for statistical analysis. Error bars indicate ±SE (n = 3). ΔΔCt method was used for qPCR analysis. Asterisks (*, ** or ***) indicate a significant difference between embryogenic cells (EC) and non-embryogenic cells (NEC) at P < 0.05, 0.01, or 0.001, respectively, by student’s t-test

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