Fig. 5

Craniofacial shape and gene expression in Arctic charr (Salvelinus alpinus) embryos at hatching and first feeding. Principal components analysis (PCA) plots showing both relative gene expression (for growth and skeletal related genes, modified [29]) and ventral craniofacial shape variation attributed to family in the brown Arctic charr morph from lake Vatnshlíðarvatn, at a) hatching (H), and b) first feeding (FF), with the effect of offspring size included (see Fig. S2 for PCA without the effect of size). Coloured shapes and associated standard error bars correspond to mean shape of offspring within a family. For gene expression only, green and red arrows show those genes indicated with the highest PCA loadings at the positive (green) and negative (red) extremes of the PC axes, with the top three genes (where applicable) listed from the highest to lowest loadings. Deformation grids at the extremes of both axes represent the amount of shape change, with two-fold magnification