Fig. 5From: Transgenic and knockout analyses of Masculinizer and doublesex illuminated the unique functions of doublesex in germ cell sexual development of the silkworm, Bombyx moriMorphological analysis of the sperm bundles of Masc-R females homozygous for BmdsxF mutation. The sperm bundles isolated from adult testes were observed under a phase-contrast microscope. a Apyrene sperm bundles from normal male animals. b, c The sperm bundles isolated from Masc-R females with BmdsxFΔ85 heterozygous (b) or homozygous mutation (c) resembled apyrene sperm bundles. d Eupyrene sperm bundles from normal male animals. e, f The sperm bundles isolated from Masc-R females with BmdsxFΔ85 heterozygous (e) or homozygous mutation (f) resembled eupyrene sperm bundles. Ap, apyrene sperm bundle; Ep, eupyrene sperm bundle. g The mean length of the sperm bundles. Error bars indicate standard deviation. * indicates a significant difference, as determined by Welch’s t-testBack to article page