Fig. 3
Influences of inhibitors of ion-transport mechanisms on Vmem in the FE. a WFM-images of typical experiments showing ArcLight-expressing S10B-follicles after incubation in either the respective inhibitor or the control solution (DMSO or ethanol; scale bars represent 200 μm); insets show enlarged examples of representative follicles in pseudocolour (arrows point to FE; scale bars represent 50 μm). Relative hyperpolarisation is indicated by stronger (bright/white), relative depolarisation by weaker (dark/blue) fluorescence intensities. The experiments were repeated at least four times. b While amiloride and verapamil caused increasing fluorescence intensities (hyperpolarisation), 9-anthroic acid, furosemide, glibenclamide and concanamycin A caused decreasing fluorescence intensities (depolarisation). To consider the variability between experiments, mean intensity ratios of the experimental and control groups (inhibited/control) of n = 4 experiments for each inhibitor were calculated. Mean values, shown with their standard deviation, were compared using a one-sample t-test (* p < 0.05; ** p < 0.01). The strongest effects on Vmem were obtained with 9-anthroic acid, furosemide and glibenclamide, respectively