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Fig. 6 | BMC Developmental Biology

Fig. 6

From: Wingless and Archipelago, a fly E3 ubiquitin ligase and a homolog of human tumor suppressor FBW7, show an antagonistic relationship in wing development

Fig. 6

Overexpression of Ago decreases Wg secretion, and loss of Ago increases Wg secretion in vitro.a Amount of Wg in conditioned media is increased by loss of Ago and decreased by gain of Ago in S2 cells. S2 cells are co-transfected with GFP-wg in combination with either ago dsRNA or myc-ago. Extracellular Wg in culture media and intracellular Wg in cell extracts were detected with both anti-GFP and anti-Wg antibodies. α-Tubulin was used as an internal control. b Level of extracellular Wg was decreased by myc-ago expression in S2 tub-wg cells

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BMC Developmental Biology

ISSN: 1471-213X

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