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Fig. 4 | BMC Developmental Biology

Fig. 4

From: Ephrin-B2 paces neuronal production in the developing neocortex

Fig. 4

Cell cycle duration and numbers of progenitors in Efnb2 cKONes. a. Transverse sections of the neocortex of EdU/BrdU-injected E13.5 control and Efnb2lox/lox; Nes-Cre embryos were immunostained for BrdU (red), Tbr2 (green) and EdU (blue). Scale bars: 25 μm. The injection protocol and the various cell populations that were counted are indicated on the right. b. S-phase duration (Ts) and cell cycle duration (Tc) were calculated using the indicated formulas. The graph shows Tc in control (n = 6) and Efnb2lox/lox; Nes-Cre embryos (n = 5). Data for each counted hemisphere are plotted. c. Quantification of AP (Tbr2-), BP (Tbr2+) and total progenitor numbers in control (n = 6) and Efnb2lox/lox; Nes-Cre embryos (n = 5). Data for each counted hemisphere and mean ± SEM are reported, unpaired t-test with Welch’s correction (*P < 0.05). d. Transverse sections of the neocortex of E14.5 control and Efnb2lox/lox; Nes-Cre embryos were immunostained for Tbr2 (red) and Pax6 (blue). Scale bars: 25 μm. e. Quantification of AP (Pax6+), BP (Tbr2+) and total progenitor numbers in control (n = 4) and Efnb2lox/lox; Nes-Cre embryos (n = 4). Mean ± SEM are reported. AP: apical progenitors; BP: basal progenitors

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