Fig. 1
From: Ephrin-B2 paces neuronal production in the developing neocortex
Ephrin-B2 is dynamically expressed in the developing neocortex. a. Efnb2 in situ hybridization on transverse sections of the neocortex at different developmental stages (indicated). Scale bar: 500 μm. b. Epifluorescence (GFP; green) detection on a transverse section of the neocortex of an E12.5 Efnb2:H2BGFP embryo. The section was immunostained with a phospho-EphB1–2 antibody (red) and Draq5 (blue). c. Western blot analysis of E13.5 neocortex tissue extracted from control (n = 5) and Efnb2lox/lox; Nes-Cre (n = 6) embryos. Antibodies are indicated on the side. Quantification of the signal (bottom) shows the ratio between pEph and total Eph