Fig. 3
Compared to wt, in grk S9, the a-p Vmem-gradient is significantly shallower, and in grk S10B, transversal Vmem- and pHi-gradients are missing in the FE. a The a-p Vmem-gradients (fluorescence intensity ratios cFC/mFC) in S9 were evaluated as described previously [7]. The a-p pHi-gradients (fluorescence intensity ratios pFE/aFE) were quantified according to Fig. 1e2. The higher the fluorescence intensity ratio is, the steeper is the gradient. Mean values (± SD, standard deviation) were compared using an unpaired t-test. Difference for Vmem-gradients is significant (*** p < 0.001); difference for pHi-gradients is not significant (pHi-gradients are shallower than Vmem-gradients). b Transversal gradients (fluorescence intensity ratios FE2/FE1; larger value vs. smaller value), and c a-p gradients (fluorescence intensity ratios pFE/aFE) in S10B were quantified as shown in Fig. 1e. Differences for transversal gradients are significant (* p < 0.05); differences for a-p gradients are not significant