Fig. 5

miRNA-1296 suppresses the expression of KPNA7 protein in HEK293 cells. a A representative western blot showing reduction of KPNA7 protein in HEK293 cells expressing miRNA-1296. ACTB was used as a loading control. The experiment was repeated three times. b ImageJ software was used to quantify the protein bands. Abundance of KPNA7 protein was normalized relative to abundance of ACTB protein. Data are expressed as mean relative pixel density (n = 3, mean ± SEM). Asterisk indicates statistical difference (P < 0.05)