Fig. 8
Schematic summary of effects of pHi and/or Vmem on bMF, and quantification of bMF-condensations (cf. Figure 7). a Alkalisation (together with hyperpolarisation or no clear effect on Vmem) caused by glibenclamide, furosemide or 9-anthroic acid prevented condensation of bMF (see b) and stabilised their parallel alignment, while the bMF-bundles became thinner. This was accompanied by enhanced angles of pHi-gradients and reduced angles of Vmem-gradients (shown on the right, cf. Figure 2). In contrast, acidification (together with no clear effect on Vmem), caused by amiloride or bafilomycin, led to an increasing area of bMF-condensation (see b) in both the a-p and the d-v direction. Moreover, bMF-bundles appeared to be thicker and more disordered. This was accompanied by reduced angles of pHi-gradients and no clear effects on angles of Vmem-gradients (shown on the right, cf. Figure 2). b Quantification of bMF-condensations in mbFC (for region of interest, see box marked in a) supported the results described above. Mean values, shown with their standard deviation, were compared with the respective controls using an unpaired t-test (3 ≤ n ≤ 8; * p < 0.05; ** p < 0.01; *** p < 0.001). Verapamil (slight alkalisation combined with strong hyperpolarisation) resulted either in condensation or in depolymerisation of bMF