Fig. 7

The bMF-organisation is affected by alterations of both pH_i and V_mem. Using inhibitors of ion-transport mechanisms, we modified pH_i and/or V_mem as well as the bMF-pattern. The results obtained with living Lifeact-GFP follicles (right columns) were similar to those obtained with fixed wild-type follicles using fluorescent phalloidin (left columns). Typical follicles of S10b are shown. For summary, see Fig. 8. Scale bars refer to all pictures in the same column. The inhibitors glibenclamide (ATP-sensitive K⁺-channels) or furosemide (Na⁺/K⁺/2Cl⁻-cotransporters), which both caused strong alkalisation (cf. Figure 2), resulted in parallel alignment of bMF in all FC (control DMSO). Glibenclamide, which led to (moderate) hyperpolarisation, stabilised the bMF-bundles, while furosemide, which had no clear effect on V_mem, caused partial disintegration of bMF. The inhibitor 9-anthroic acid (Cl⁻-channels), which resulted in slight alkalisation and no clear effect on V_mem (cf. Figure 2), also reduced the frequency of bMF-condensations (control ethanol). On the other hand, bafilomycin (V-ATPases) or amiloride (Na⁺/H⁺-exchangers, Na⁺-channels), both acidifying inhibitors with no strong impact on V_mem (cf. Figure 2), led to an increasing area of bMF-condensation followed by disintegration of bMF in cFC and dorsal mbFC