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Fig. 7 | BMC Developmental Biology

Fig. 7

From: YWHA (14-3-3) protein isoforms and their interactions with CDC25B phosphatase in mouse oogenesis and oocyte maturation

Fig. 7

a In vitro maturation of oocytes from Ywhah and Ywhae oocyte-specific and global knockout females. For each female of a given genotype, the total number of oocytes examined is indicated in parentheses and the percent of those oocytes that underwent germinal vesicle breakdown (GVBD) is indicated in the bar graph. The extent of oocyte maturation, as indicated by GVBD, is high in females of all genotypes in which Ywhah or Ywhae is inactivated specifically in the oocyte or globally. b Representative images of oocytes collected from knockout females and the corresponding images of the mature eggs the following day after in vitro maturation of oocytes from knockout females and wild-type females. Oocytes from females of all genotypes appear normal with intact germinal vesicles. Arrows indicate the first polar body of some eggs, which is not always visible because of the orientaion of the egg. Scale bars represent 50 μm. c In vitro maturation of oocytes from females in which both the Ywhah and Ywhae genes were inactivated in the oocyte, oocytes from females in which one allele of Ywhah or Ywhae was inactivated (hetetrozygous), or oocytes from wild-type females

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