Fig. 6

Breeding of Ywhah and Ywhae oocyte-specific and global gene knockout mice with wild-type males. The number of pups for each female of a given geneotype is indicated for the first two litters. In vivo breding illustrates the range of offspring number in mice of different females mated with different wild-type males. Here, the number of pups produced by females with oocyte-specific inactivation of Ywhah or Ywhae and global inactivation of Ywhah (a) falls within the normal range of litter sizes of mice without gene inactivation (here mice containing ZP3 or ACTB Cre, and mice with floxed Ywhah or Ywhae gene (LoxP/LoxP) and no Cre (b). However, females with global inactivation of Ywhae did not produce pups (last group in (a), see Results and Discussion)