Skip to main content
Fig. 2 | BMC Developmental Biology

Fig. 2

From: YWHA (14-3-3) protein isoforms and their interactions with CDC25B phosphatase in mouse oogenesis and oocyte maturation

Fig. 2

Expression of fluorescently-tagged YWHAH and YWHAE in mouse oocytes. Immature oocytes from wild type ICR(CD-1) mice were microinjected with mCherry-YWHAH (a) or EGFP-YWHAE mRNA (b). The corresponding brightfield images are shown at the right and the oocyte nucleus is outlined with a dashed black line. The images are representative of results from 16 different oocytes expressing mCherry-YWHAH from five different experiments and 12 oocytes expressing EGFP-YWHAE from three different experiments. c Representative images of three oocytes simultaneously injected with mCherry-YWHAH and EGFP-YWHAE mRNA. Both isoforms are expressed in the same cell and the merged emission channels suggests a very similar distribution for both isoforms. The white squares shown in Cell 2 are the regions of interest used to determine the average Pearson’s correlation coefficient for this cell (see Results). The nucleus was imaged at higher magnification in Cell 3. There is a clear distinction between the cytoplasm and the nucleus, with the nucleus displaying less fluorescence than the cytoplasm and the nucleolus displaying little fluorescence. YWHAH and YWHAE are primarily cytoplasmic in an immature oocyte. All of seven other injected cells were similar. The scale bars represent 10 μm

Back to article page