Fig. 3
From: Role of PRY-1/Axin in heterochronic miRNA-mediated seam cell development
pry-1 is necessary for normal miRNA expression in both C. elegans and C. briggsae. (a) Volcano plot of differentially expressed miRNA genes in pry-1(mu38). Red and green dots mark significantly altered upregulated and downregulated transcripts, respectively, with a p-adj value of < 0.05. Orange dots mark transcripts that are not significantly altered (p-adj > 0.05). (b) Data showing log2 fold expression of miRNAs from RNA-Seq analysis. (c, d) qRT-PCR analysis of miRNAs in Cel-pry-1(mu38) and Cbr-pry-1(sy5353) animals at the L1 stage showed upregulation of all but miR-246. Each data point represents the mean of two replicates and error bar represents the SEM, **p < 0.01. (e, f) miR-246(n4636) mutants exhibit alae defect in the form of gaps (scale bar 0.01 mm) (e) but show no change in the number of seam cells (f). Each data point represents the mean of at least two replicates (each batch with at least 20 worms) and error bar represents the STD