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Fig. 7 | BMC Developmental Biology

Fig. 7

From: Importance of prostate androgen-regulated mucin-like protein 1 in development of the bovine blastocyst

Fig. 7

Effects of PARM1 antisense oligonucleotide GapmeR and tunicamycin at day 5.5 after fertilization on competence of embryos to become a blastocyst at day 7.5 after fertilization (Experiment 4). Data are least-squares means ± SEM. At day 5.5 after fertilization, 2.5 μL of medium in the culture drop was replaced with 2.5 μL SOF-BE2 containing vehicle (Control; solid bars) or tunicamycin (0.5 μg/mL tunicamicyn so that the final concentration would be 0.05 μg/mL; hatched bars). Treatment with tunicamycin decreased (P = 0.0001) the percent of presumptive zygotes becoming a blastocyst at day 7.5 after fertilization. There was no effect of GapmeR treatment or interaction between effects of tunicamycin treatment and GapmeR treatment on blastocyst development

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BMC Developmental Biology

ISSN: 1471-213X

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