Fig. 2

Subunits of the Vg and their fluctuation in the tested part of development of the P. tepidariorum. Visualization of three subunits (250 kDa, 47 kDa and 30 kDa) of the Vgs (arrows) in the homogenate from: eggs (1), whole body extract of males after mating (2), ovaries from females on day 38 after leaving a cocoon (3), day 40 after leaving a cocoon (4), day 43 after leaving a cocoon (5) by SDS-PAGE electrophoresis (a) and Western blot (b). Fluctuations in the Vg proteins [average ± SD] in: (c) the midgut glands, hemolymph and ovaries of the female spiders in different ages. Lowercase letters indicate significant differences within the same tissue (Tukey’s multiple comparisons test, p ≤ 0.05)