Fig. 4

Cell types and brain regions represented in early brain organoid development. a Immunostaining of 30-day-old organoids grown in the orbital shaker for FOXG-1, PAX-6, islet-1, and OTX-2. Bars = 500 μm; b Stereoscopic image of an organoid with pigmented regions. Bar = 1 mm. c Pigmented regions (box) of organoids after 45 days in culture. Bar = 1 mm. The pigmented regions (box) were positive for glycogen synthetase. Bar = 500 μm