Fig. 5

Generation of dmrt2a and dmrt2b mutant lines. a Representation of dmrt2a and dmrt2b genes. Depiction of the binding site of the three different TALEN pairs (P₁, P₂ and P₃). b Representation of the mutations obtained. In bold caps is represented the start codon, in green the indels and in orange the 3’end of the 5’UTR. c Alignment between the wildtype sequence of Dmrt2a and Dmrt2b proteins and the expected sequence in the different mutants obtained. In purple is represented the DM domain and in blue the position of putative alternative start codons. In the DM domain of Dmrt2a wildtype protein, the crucial amino acid residues for nuclear translocation and DNA-binding are highlighted, as described [13]: in underlined bold lowercase are represented the ligand-binding residues from the first zinc-binding site and, in underlined bold capital letters the residues from the second zinc-binding site. The two strongly basic residues (K77 and R78) required for nuclear translocation are shaded in yellow. UTR: Untranslated Region, DM: Doublesex and Mab3