Table 1 Genetic analysis of pcn-1 fertility. A) Thirty-one self-progeny of heterozygous pcn-1(am315flag)/+ hermaphrodites were analyzed by PCR of single animals to determine genotype after egg-laying was complete. Full genotypes: pcn-1(+/+), pcn-1(am315/+), and pcn-1(am315/am315). Hermaphrodites were scored as “fertile” if they deposited one or more eggs on the agar surface that hatched and developed into larvae. Animals were scored as “sterile” if they deposited no eggs on the agar surface, or if all deposited eggs failed to hatch. B) The percent of eggs that generate larvae and adults was determined for pcn-1(+/+); fog-2(q71/+) and pcn-1(am315flag/+); fog-2(q71/+) hermaphrodites, as described in the methods. n refers to total number of eggs analyzed. A chi-square test was used to compare the larval and adult survival. The total self-fertile brood size was determined as described in the methods. ANOVA was used to compare the brood size. Mean ± s.d. n refers to total number of parental worms analyzed. NS indicates P > 0.01, * P < 0.01, ** P < .001, *** P < .0001

From: Cell cycle accumulation of the proliferating cell nuclear antigen PCN-1 transitions from continuous in the adult germline to intermittent in the early embryo of C. elegans