Fig. 6

Detection of zygotic vas4 transcripts in PGCs. In situ hybridization with a DIG-labeled RNA probe detected no signals in embryos at early gastrulation stage (a, b, vegetal views). Shortly after gastrulation was completed vas4 transcripts were detected in one or two cells (c, d). At tailbud stage (e) and newly hatched larva (f), signals were observed in the posterior trunk. The position of vas4-positive cells corresponds to the location of PGCs. Scale bar: 50 μm