Fig. 3
From: Expression and function of microRNA-9 in the mid-hindbrain area of embryonic chick
OTX2, GBX2, WNT1 and EN1 expressions were not disturbed by ectopic miR-9 expression. Lateral view of the neural tube at MH level overexpressing miR-9 (a, b, d, e, j, k, m, n, s, t), miR-9-LNAi (g,h,m,n,) or pCAX-EGFP (p,q). Left brain halves served as controls (c,f,i,l,o,r,u). Brains were electroporated at HH9–10 and fixed after 20-24 h. ISH for OTX2 and GBX2 (a, c), WNT1 (d,f,g,i), EN1 (j,l,m,o,p,r) and HES1 (s,u). Electroporated cells were visualised with an immunostaining for EGFP (b,e,h,k,n,q,t). In (g,i) Fast Red was used to visualise WNT1 with a red fluorescence. Ectopic miR-9 or miR-9-LNAi expression did not change the expression pattern of OTX2 and GBX2 (a), WNT1 (d,g), EN1 (j,m) or HES1 (s). Abbreviations: Di-diencephalon, Mes-mesencephalon, MHB-mid-hindbrain boundary, Rh-rhombencephalon