Phylogeny, expression patterns and regulation of DNA Methyltransferases in early development of the flatfish, Solea senegalensis

Table 3 Summary of expression results for the four dntms in sole. Results obtained about gene expression patterns as determined by qPCR and WISH in embryos, larvae and juveniles and their regulation by 5-AzaC and temperature

	Expression patterns^a				Regulation^b
	Embryos	Lecitotrophic larvae	WISH patterns in larvae	Juveniles	5-AzaC	Temp
dnmt1	--	↓↓	Brain, spinal cord, eyes, pharynx and intestine	Brain	↓	---
dnmt3aa	↑↑	↓	Somites, pronephric duct, brain, spinal cord, eyes, pharynx and intestine	Spleen and gills	↑	↑↑
dnmt3ab	↑	↑	Brain, eye layers, spinal cord and intestine	Heart and brain	--	↑
dnmt3bb.1	↓	↓	Brain, pectoral fin buds, pronephric duct, eyes, intestine, pharynx and branchial arches	Gills	--	---

^aThe increase (↑) or decrease (↓) of gene expression from gastrula to hatch in embryos or during lecitotrophic larval development is shown
^bThe up- (↑) or downregulation (↓) of gene expression after 5-AzaC treatments or in response to low temperature (16 °C) is shown
The arrows indicate if expression is differentially induced (↑ = or reduced (↓) for each factor and their number denote the intensity of the response

ISSN: 1471-213X