Table 1 Sequences of primers used in the present study
Primer Name F—Forward/R—Reverse | DNA-Sequence 5′-3′ | Annealing Temperature (°C) | Fragment Size (bp) |
|---|---|---|---|
Gene-specific Primer pairs for RT-qPCR | |||
GnRH-R2-F | 5′-CGTGCGGTGAAGGCGAAGGGGGTGG-3′ | 60.7 | 387 |
GnRH-R2-R | 5′-ACACAACCCCAACTAAGCAAGCATA-3′ | 64.7 | |
18sRNA primers | |||
18sRNA-R | 5′- TGATTGGGACTGGGGATTGAA-3′ | 59.2 | 232 |
18sRNA-F | 5′- TAGCGACGGGCGGTGTGT-3′ | 62.4 | |