Fig. 5

GnRH-R2 locations in the brain and ovary tissues. Immunohistochemical positive signals of GnRH-R2 immunolabeling are shown in brown. B1–B3: whole brain sections stained with H&E; B4–B5: GnRH-R2 immunolabels in different parts of the brain; and NB: negative control for brain. O1–O3: whole ovary sections stained with H&E; O4–O5: GnRH-R2 immunolabels in different parts and oocyte developmental stages of the ovary for IHC (O5 provides a more magnified view of the ovary IHC signal); and NO: negative control for the ovary tissue. Key: OB, olfactory bulb; SG, stratum granulare; NC, Neurogliocyte, pOc, primary oocyte; sOc, secondary oocyte; Oc, oocyte; N, oocyte nucleus; and Cp, oocyte cytoplasm. Scale bar = 100 μm