Fig. 2

Loss of Grhl3 leads to significant differences in skull length and width. a-d Schematic diagrams on WT skulls showing measurements taken using Micro CT analysis. Micrographs highlighting the morphometric landmarks utilised for quantitative analyses, shown in sagittal (a) and ventral (b) views (see methods for details). Representative diagrams showing measurements taken, namely sagittal length (1–3), cranial vault length (2–3) and width (6–7), and cranial height (4–5; c, d). e, f Representative 2D images generated of the skull with the caudal sections removed digitally, allowing clear visualisation of the frontal (f) and parietal (p) bones, and the suture between them. (g-k) Quantitation of measurements of regions outlined in (a-d), namely maximal sagittal length (g), cranial length (h), maximal cranial width (i), cranial height (j), and the open suture area (k) in WT and Grhl3 ^−/− embryos. *p < 0.05; **p < 0.01; ***p < 0.005