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Fig. 3 | BMC Developmental Biology

Fig. 3

From: Relating proton pumps with gap junctions: colocalization of ductin, the channel-forming subunit c of V-ATPase, with subunit a and with innexins 2 and 3 during Drosophila oogenesis

Fig. 3

Ductin and Inx2 are partly colocalized in plasma membranes and in the cytoplasm of follicle cells and germ-line cells, but often in different plaques. a to c: Ductin; a’ to c’: Inx2; A”: merged image, ductin (red), Inx2 (green) and colocalization of both (yellow), WFM. Nuclei were stained with DAPI. No specific staining was obtained with NIS (cf. inset in Fig. 4b). In previtellogenic follicles (a to a”, stage 6; b to b’, stage 7), ductin and Inx2 co-occur in lateral and apical follicle-cell membranes (aFCM) and in the cytoplasm of FC, nurse cells (NCN, NC nucleus) and the oocyte (Ooc). While prominent cytoplasmic particles or vesicles containing Inx2 can be observed in germ-line cells (a’, b’), ductin is found to be enriched in NC membranes (NCM), in the oolemma (Ool), in anterior and posterior polar cells (aPC, pPC) and in stalk cells (SC). In vitellogenic follicles (c to c’, stage 10b), ductin and Inx2 co-occur in lateral and apical FC membranes (FCM), while ductin is found to be enriched in pPC, and Inx2 in the Ool, respectively. Both proteins are concentrated in lateral FCM, but they often localize in different plaques (cf. arrowheads in c and c’). Scale bars, 10 μm

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