Fig. 1

Schematic drawing and lectin labelling of Macrostomum lignano. a Schematic drawing of an adult specimen. b-i Confocal projections of lectin labelling imposed on DIC images. b SBA and (c) GSL I staining of frontal glands 1, 2, and 4, pharyngeal glands, and the antrum. b SBA additionally stains the cement glands. d sWGA staining of frontal glands 3 and pharyngeal glands. e RCA labelling. Arrowheads indicate the unstained epidermal layer. f LCA staining of epidermal junctions. Inset shows the epidermis at a higher magnification. g Ubiquitous Con A labelling of the epidermis. h PHA E and (i) PHA L staining of the glycocalyx of the epidermal microvilli. Inset illustrates stained microvilli of adhesive organs. An antrum, ao adhesive organs, cg cement glands, egg developing egg, fg frontal glands, ov ovaries, ts testes, ph pharyngeal glands. Scale bars: 100 μm