Fig. 3

Effect of EGF on mPdx1 transduction. The expression of human pancreatic marker genes by mPdx1-transduced hAECs in a culture environment lacking EGF was assayed by means of qPCR over a 7 day period. (a) Gene expression in cells grown in the absence of EGF for 2 days (b) Gene expression in cells grown in the absence of EGF for 7 days (c) Comparison of gene expression in cells grown in the absence of EGF for 2 and 7 days. Fold change was compared to cells grown in the presence of 10 ng/mL EGF. Although it appeared that there was a loss of gene expression across the panel by the 7th day, this loss was not significant when compared to expression in cells grown in an environment containing EGF. (* p < 0.05, ** p < 0.005, *** p < 0.0005; n = 2). Error bars represent mean ± SD