Fig. 2
Phenotypes of ALK3 and ALK6 morphant embryos. Embryos were injected with 0.8 pmol antisense morpholino in both blastomeres at the 2-cell stage as indicated and cultured till NF stage 41. a Normal NF stage 41 embryos after injection of an unrelated control Morpholino oligonucleotide (control MO). b Embryos injected with either ALK3 or ALK6 translation blocking Morpholino oligonucleotides (ALK3 MO and ALK6 MO respectively) showing defects in the development of ventral fins. c In the retina of a subset of either ALK3 MO or ALK6 MO injected embryos un- or less pigmented patches were observed. d Dorsalized ALK3 morphant embryo. e ALK6 morphant embryo showing increased pigmentation of the head and narrower gills. f In most cases ALK3 or ALK6 morphant embryos exhibited complex phenotypes with defects in the ventral fin, retina pigmentation, smaller or absent eyes, aberrant pigmentation and loss of cranial cartilage. Black arrowheads indicate eye phenotypes, white arrows missing ventral structures and black arrows mark the cranial cartilage. g Phenotype frequencies from at least three independent experiments are summarized in the graph. Differences between indicated groups were analyzed using the Χ 2 test. Statistically significant deviations (p-value < 0.01) are indicated by double asterisks; n.s.: not significant