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Fig. 2 | BMC Developmental Biology

Fig. 2

From: Intrinsic factors and the embryonic environment influence the formation of extragonadal teratomas during gestation

Fig. 2

Pluripotency factor expression in the somitogenesis stage embryo. a Klf4 mRNA expression in an E10.5 mouse embryo analysed by wholemount in situ hybridisation. (a’) Magnified detail from image a’ showing the increased presence of Klf4 transcripts at the posterior. (a”) Embryo section showing Klf4 expression within the chordoneural hinge (mCNH) of the E10.5 tailbud. pCNH, region immediately posterior to CNH. (a”’) Inset: Klf4 in situ hybridisation in undifferentiated ES cells (b) qPCR analysis of the expression of the main pluripotency factors and positive control genes (T(Bra) and Evx1) in CNH regions microdissected from an E10.5 (32–35 somite) embryo (their locations indicated  in a”). Expression levels are represented as relative to TBP. Error bars represent s.d. Mouse ES cells were included as a positive control

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