Fig. 3From: Cooperative and independent functions of FGF and Wnt signaling during early inner ear developmentExpression patterns of genes encoding Wnt ligands and receptors are unchanged in Spry1 −/− ; Spry2 −/− embryos. a – j In situ hybridization analyses of genes encoding Wnt ligands and receptors in Spry1 −/− ; Spry2 −/− embryos and controls at OEPD or early otic placode stages. Lateral views of whole-mount embryos are shown; embryos are oriented as indicated. Pre-otic regions are bracketed. k Semi-quantitative reverse-transcriptase PCR analyses of candidate Wnt and Fzd transcript levels in RNA collected from 5 – 7 s hindbrain and OEPD-containing tissue microdissected from the genotypes indicated. Wnt genes (Wnt1, Wnt3a, Wnt6, and Wnt8a) with known expression adjacent to the OEPD are controls. Scale bar, 100 μmBack to article page