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Fig. 3 | BMC Developmental Biology

Fig. 3

From: Sox7 is dispensable for primitive endoderm differentiation from mouse ES cells

Fig. 3

Generation of Sox7-null ES cells. (a) Design of the gene targeting vector. Sox7 coding region including exon 1 and 2 were replace by PGK-pac∆TK casette flanked by loxP and lox2722. (b) Correctly targeted clone (S7mt1) was confirmed by Southern blot. (c) Genotyping results of high dose puromycin resistant clones by Southern blot. First five lanes are clones from S7mtG1 line (used in Fig. 5 and 6) and last three lanes are from S7mt1 line. We obtained three null lines from five S7mtG1 high dose puromycin resistance clones and two from six S7mt1 derivatives (half of the results is shown here)

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