Fig. 9
From: Key pathways regulated by HoxA9,10,11/HoxD9,10,11 during limb development
Immunofluorescent staining of E15.5 Wild type HoxAADD (a–d) and mutant Hoxaadd (Hoxa9,10,11 −/−;Hoxd9,10,11 −/−) (e–h) forelimbs. Arrowhead: radius, Arrow: ulna, the autopod is oriented to the left of the image. a and e: Six2 immunostaining, showing an increased expression in mutant chondrocytes. b and f: Gas1 staining, showing an increase in mutant limbs that is restricted to cells flanking chondrocytes, consistent with the inclusion of some perichondrial cells in the LCM samples. c and g: Lef1 staining, showing an absence of staining in mutant chondrocytes. d and h: Runx3 staining, showing an absence of staining in mutant chondrocytes