Fig. 4

Knockdown of HP1γ in male germ cells impacts processes related to mitosis and meiosis. a. Affymetrix whole genome gene expression analysis was performed on GC1 HP1γ knockdown cells compared to scrambled shRNA control cells. 273 genes targets are significantly (fold change ±1.25, p < 0.005) activated or repressed in the absence of HP1γ. b. Gene Ontology (GO) ANOVA analysis of the 273 targets was performed and revealed significant (p < 0.05) enrichment of mitosis and meiosis associated processes, as well as processes involved in differentiation. c. qPCR validation of a subset of identified knockdown targets with known function in male fertility is shown. Fold change of shHP1γ compared to shCTRL expression is represented on a scale of ±2 and shown next to the corresponding Affymetrix data. d. The top-scoring Ingenuity-based network analysis network is significantly (p < 0.05) associated with cellular development, gene expression, and cell cycle