Fig. 1
From: The Aurora A-HP1γ pathway regulates gene expression and mitosis in cells from the sperm lineage
P-S83-HP1γ is present in testicular tissue and spermatozoa. a. P-S83-HP1γ in normal mouse testicular tissue. Immunohistochemistry of P-S83-HP1γ (left panel) reveals that the cell population near the basal lamina, typically undergoing high levels of proliferation, is the most highly P-S83-HP1γ positive. Right panel demonstrates negative control of immunohistochemistry staining with secondary antibody only. Scale bar represents 50 μM. b and c. P-S83-HP1γ localization in spermatozoa by electron microscopy. Immunogold electron microscopy depicting mature human spermatozoa. P-S83-HP1γ was concentrated in the centriole (b) and mid-piece (c, arrow) of the spermatozoa. d and e. P-S83-HP1γ localization spermatozoa by immunofluorescence. Representative image for protein localization of human spermatozoa with immunofluorescence. Localization of P-S83-HP1γ is observed adjacent to the sperm nuclei (counterstained with DAPI in d), corresponding to the mid-piece of mature human sperm. This signal also co-localizes with centrin-2 (red in e), structural component of the centrosome located in the mid-piece