Figure 6From: An optimised whole mount in situ hybridisation protocol for the mollusc Lymnaea stagnalis Our WMISH protocol is suitable for fluorescent signal detection. The expression of COE (A), engrailed (B) and beta tubulin (C and D) in larvae treated with 0.5% SDS was detected using the fluorescent substrate Fast Red (A-D). Panel E is a scanning electron micrograph of a 62 hours post first cleavage (hpfc) old larva with the approximate localisation of the COE expression indicated in red. Panel F is a lateral perspective of a 90 hpfc old larva with indicated engrailed expression. Panels G and H show the positions of beta tubulin expression in a 57 hpfc old larva (G, ventral perspective) and a 100 hpfc old larva (H, lateral perspective). Panels B-D are projections of confocal laser scanning micrographs. Panels B, D and H are reflected about the vertical axis for consistency of presentation. All scale bars are 50 μm.Back to article page