Figure 1

Expression of type II DGK mRNAs (A) and proteins (B) in the reproductive organs: testis, ovary and uterus. (A) RT-PCR analysis of the mRNA from the reproductive organs was performed: testes from 12-week-old male mice and ovaries and uteruses from 12-week-old female mice. The panels show that 999-bp cDNA fragments were amplified for DGKδ (35 cycles), 826-bp cDNA fragments were amplified for DGKη (35 cycles), 842-bp cDNA fragments were amplified for DGKκ (35 cycles) and 978-bp cDNA fragments were amplified for mouse glyceraldehyde phosphate dehydrogenase (GAPDH, 35 cycles), as determined through agarose gel electrophoresis. The asterisk indicates a ~730-bp band that was amplified from the testis mRNA. Representatives of five independent experiments with five male and five female mice are shown. (B) The protein samples (30 μg) from the indicated tissues of 10-week-old male and female mice were detected by Western blotting using anti-DGKδ and DGKη antibodies. Representatives of five independent experiments with five male and five female mice are shown.