Figure 1

DIAP1 loss of function mutation results in altered cell polarity and adhesion. (A-F) Apoptosis in th¹⁰⁹ heterozygous and homozygous embryos; (A-C) th¹⁰⁹ heterozygous embryo stained for fragmented DNA (TUNEL) (A), activation of the executioner caspase drICE (B) and expression of ftz::lacZ (B); the ftz::lacZ transgene results in a striped expression pattern of beta-galactosidase (beta-gal) and indicates a copy of the wild type allele of the DIAP1 gene. Arrowheads in (A-C) indicate apoptotic cells in the head region. (D-F) th¹⁰⁹ homozygous embryo stained for fragmented DNA (D) and expression of ftz::lacZ and active drICE (E). (C, F) Merged images show anti beta-galactosidase and anti active-drICE staining in red and TUNEL signal in green/yellow. (G-R) th¹⁰⁹ mutant embryos stained for Bazooka (Baz, in blue) (H, L, P), Neurotactin (Nrt, red) (I, M, Q) and Dα-Catenin (Dα-Cat, green) (J, N, R). (G-J) th¹⁰⁹ heterozygous embryo; (K-N) th¹⁰⁹ homozygous embryo 45 min after cephalic furrow formation. (O-R) th¹⁰⁹ homozygous embryo 60 min after cephalic furrow formation. Scale bars in (F) 40 μm valid for (A-F, G, K, O); scale bar in (R) 10 μm for (H-J, L-N, P-R).